Combocat
Scalable and interpretable drug combination screening
Welcome
This is the documentation for Combocat, a start-to-finish platform for drug combination screening.
In this documentation, you will find steps to complete both experimental and analytical parts of Combocat experiments. The experimental sections include step-by-step protocols with examples. In the data analysis sections, we provide steps for processing and analyzing results using the combocat R package. Browse links in the sidebar to the left to find out information about each step.
Use the search function in the side bar to look for specific protocol steps or topics
Overview
Drug combinations are critical to conditions like cancers and infectious diseases, but finding new combinations is a slow and challenging process. The scale of a combination experiment increases rapidly with each tested drug, and testing many combinations has traditionally been impractical.
Combocat is a method for scalable drug combination screening which uses acoustic liquid handler protocols and custom drug template designs for fast, efficient screening. Everything has been designed to work in an end-to-end fashion. This means a researcher can test 1, hundreds, or even thousands of drug combinations from start to finish.
Critically, Combocat also provides a complete framework for analyzing the experimental data. Analysis is straightforward and provides interpretable publication-quality figures.
Combocat is fully open-source!🎉
Reference
Please cite our published work as:
Wright, W.C., Pan, M., Phelps, G.A. et al. An open-source screening platform accelerates discovery of drug combinations. Nat Commun 16, 11005 (2025). https://doi.org/10.1038/s41467-025-66223-8.
📝Full PDF: https://rdcu.be/eUIln